Intern
  • Pipettierroboter
Core Unit Systemmedizin

Single cell processing

Becton Dickinson FACS Aria III (courtesy of the Institute of Molecular Infection Biology)

  • ca. 1 x 96 well plate / 1 min
  • Lasers: 405 nm, 488 nm, 561 nm, 633 nm
  • Nozzles: 70 nm, 85 nm, 100 nm

10x Genomics Chromium Controller

  • Droplet-based single cell partitioning
  • 500-80.000 cells per chip
  • Compatible protocols: 3', 5' Gene Expression, ATACseq, Multiome
  • Capture rate for cells loaded: 65%