Functional genomics and transcriptomics

Our facility provides access to Next Generation Sequencing (NGS) technologies for molecular high-throughput investigations of RNA and DNA:

RNA-Sequencing (RNA-Seq for Gene Expression- and Trancriptome Analysis)

• mRNA-Sequencing with Oligo-dT-capture beads (Eukaryotes)
• total RNA-Sequencing with Random Primer
• total RNA-Sequencing after Depletion of Globin-mRNA or rRNA (Prokaryotes and Eukaryotes)
• small RNA-Sequencing, i.a. for bacterial transcriptomes
• Dual-RNA-Sequencing of Prokaryote- and Eukaryote-RNA (human, mouse)

Whole-Genome-Sequencing (WGS), i.a. bacterial and viral genomes

Amplicon-Seq (targeted sequencing of PCR-Amplicons, e.g., with Phase-Shift-Primer)

RIP-Sequencing (RIP-Seq – analysis of protein interactions with RNA)

CLIP-Sequencing (CLIP-Seq – analysis of protein interactions with RNA-binding motives after UV-cross-lining)