Cell Sorting and Immune-Monitoring

Core Unit Fluorescence-activated Cell Sorting and Immune-Monitorin

Summary

Electronic cell sorting based on multi parameter flow cytometry have become essential methods in biomedical research. This unit offers full service in the field of FACS, it provides support in designing proper cell preparation and staining protocols suitable for the further experiments. The helpdesk platform for analytical and preparative methods is managed by an expertised operator with longtime experience in flow-cytometry and cell preparation.
This unit is open for all scientists necessitating very pure populations even out of rare events and valid FACS data. It is equiped with state of the art analyzers and cell-sorters.
 

Selected Publications

Ullrich, E., Abendroth, B., Rothamer, J., Huber, C., Buttner-Herold, M., Buchele, V., Vogler, T., Longerich, T., Zundler, S., Volkl, S., et al. (2018). BATF-dependent IL-7RhiGM-CSF+ T cells control intestinal graft-versus-host disease. J Clin Invest 128, 916-930.

Fichtner, A.S., Karunakaran, M.M., Starick, L., Truman, R.W., and Herrmann, T. (2018). The Armadillo (Dasypus novemcinctus): A Witness but Not a Functional Example for the Emergence of the Butyrophilin 3/Vgamma9Vdelta2 System in Placental Mammals. Front Immunol 9, 265.

Muhammad, K., Rudolf, R., Pham, D.A.T., Klein-Hessling, S., Takata, K., Matsushita, N., Ellenrieder, V., Kondo, E., and Serfling, E. (2018). Induction of Short NFATc1/alphaA Isoform Interferes with Peripheral B Cell Differentiation. Front Immunol 9, 32.

Borst, A., Haferkamp, S., Grimm, J., Rosch, M., Zhu, G., Guo, S., Li, C., Gao, T., Meierjohann, S., Schrama, D., et al. (2017). BIK is involved in BRAF/MEK inhibitor induced apoptosis in melanoma cell lines. Cancer Lett 404, 70-78.

Dagvadorj, N., Deuretzbacher, A., Weisenberger, D., Baumeister, E., Trebing, J., Lang, I., Kochel, C., Kapp, M., Kapp, K., Beilhack, A., et al. (2017). Targeting of the WT191-138 fragment to human dendritic cells improves leukemia-specific T-cell responses providing an alternative approach to WT1-based vaccination. Cancer Immunol Immunother 66, 319-332.

Garcia-Guerrero, E., Gogishvili, T., Danhof, S., Schreder, M., Pallaud, C., Perez-Simon, J.A., Einsele, H., and Hudecek, M. (2017). Panobinostat induces CD38 upregulation and augments the antimyeloma efficacy of daratumumab. Blood 129, 3386-3388.

Gogishvili, T., Danhof, S., Prommersberger, S., Rydzek, J., Schreder, M., Brede, C., Einsele, H., and Hudecek, M. (2017). SLAMF7-CAR T cells eliminate myeloma and confer selective fratricide of SLAMF7(+) normal lymphocytes. Blood 130, 2838-2847.

Klein-Hessling, S., Muhammad, K., Klein, M., Pusch, T., Rudolf, R., Floter, J., Qureischi, M., Beilhack, A., Vaeth, M., Kummerow, C., et al. (2017). NFATc1 controls the cytotoxicity of CD8(+) T cells. Nat Commun 8, 511.

Effenberger, M., Bommert, K.S., Kunz, V., Kruk, J., Leich, E., Rudelius, M., Bargou, R., and Bommert, K. (2017). Glutaminase inhibition in multiple myeloma induces apoptosis via MYC degradation. Oncotarget 8, 85858-85867.

Giampaolo, S., Wojcik, G., Serfling, E., and Patra, A.K. (2017). Interleukin-2-regulatory T cell axis critically regulates maintenance of hematopoietic stem cells. Oncotarget 8, 29625-29642.

Bergfeld, A., Dasari, P., Werner, S., Hughes, T.R., Song, W.C., Hortschansky, P., Brakhage, A.A., Hunig, T., Zipfel, P.F., and Beyersdorf, N. (2017). Direct Binding of the pH-Regulated Protein 1 (Pra1) from Candida albicans Inhibits Cytokine Secretion by Mouse CD4(+) T Cells. Front Microbiol 8, 844.

Monjezi, R., Miskey, C., Gogishvili, T., Schleef, M., Schmeer, M., Einsele, H., Ivics, Z., and Hudecek, M. (2017). Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors. Leukemia 31, 186-194.

Muller, E., Bauer, S., Stuhmer, T., Mottok, A., Scholz, C.J., Steinbrunn, T., Brunnert, D., Brandl, A., Schraud, H., Kressmann, S., et al. (2017). Pan-Raf co-operates with PI3K-dependent signalling and critically contributes to myeloma cell survival independently of mutated RAS. Leukemia 31, 922-933.

Ribechini, E., Hutchinson, J.A., Hergovits, S., Heuer, M., Lucas, J., Schleicher, U., Jordan Garrote, A.L., Potter, S.J., Riquelme, P., Brackmann, H., et al. (2017). Novel GM-CSF signals via IFN-gammaR/IRF-1 and AKT/mTOR license monocytes for suppressor function. Blood Adv 1, 947-960.

Vaeth, M., Maus, M., Klein-Hessling, S., Freinkman, E., Yang, J., Eckstein, M., Cameron, S., Turvey, S.E., Serfling, E., Berberich-Siebelt, F., et al. (2017). Store-Operated Ca(2+) Entry Controls Clonal Expansion of T Cells through Metabolic Reprogramming. Immunity 47, 664-679 e666.

Chopra, M., Biehl, M., Steinfatt, T., Brandl, A., Kums, J., Amich, J., Vaeth, M., Kuen, J., Holtappels, R., Podlech, J., et al. (2016). Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med 213, 1881-1900.

Hollmann, C., Werner, S., Avota, E., Reuter, D., Japtok, L., Kleuser, B., Gulbins, E., Becker, K.A., Schneider-Schaulies, J., and Beyersdorf, N. (2016). Inhibition of Acid Sphingomyelinase Allows for Selective Targeting of CD4+ Conventional versus Foxp3+ Regulatory T Cells. J Immunol 197, 3130-3141.

Rosenbaum, C., Schick, M.A., Wollborn, J., Heider, A., Scholz, C.J., Cecil, A., Niesler, B., Hirrlinger, J., Walles, H., and Metzger, M. (2016). Activation of Myenteric Glia during Acute Inflammation In Vitro and In Vivo. PLoS One 11, e0151335.

Schulze, M., Fedorchenko, O., Zink, T.G., Knobbe-Thomsen, C.B., Kraus, S., Schwinn, S., Beilhack, A., Reifenberger, G., Monoranu, C.M., Siren, A.L., et al. (2016). Chronophin is a glial tumor modifier involved in the regulation of glioblastoma growth and invasiveness. Oncogene 35, 3163-3177.

Bartmann, C., Junker, M., Segerer, S.E., Hausler, S.F., Krockenberger, M., and Kammerer, U. (2016). CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) Cells: Rare Populations in the Human Decidua with Characteristics of MDSC. Am J Reprod Immunol 75, 539-556.

Busch, R., Murti, K., Liu, J., Patra, A.K., Muhammad, K., Knobeloch, K.P., Lichtinger, M., Bonifer, C., Wortge, S., Waisman, A., et al. (2016). NFATc1 releases BCL6-dependent repression of CCR2 agonist expression in peritoneal macrophages from Saccharomyces cerevisiae infected mice. Eur J Immunol 46, 634-646.

Dotterweich, J., Tower, R.J., Brandl, A., Muller, M., Hofbauer, L.C., Beilhack, A., Ebert, R., Gluer, C.C., Tiwari, S., Schutze, N., et al. (2016). The KISS1 Receptor as an In Vivo Microenvironment Imaging Biomarker of Multiple Myeloma Bone Disease. PLoS One 11, e0155087.

Groh, J., Ribechini, E., Stadler, D., Schilling, T., Lutz, M.B., and Martini, R. (2016). Sialoadhesin promotes neuroinflammation-related disease progression in two mouse models of CLN disease. Glia 64, 792-809.

Klein-Hessling, S., Rudolf, R., Muhammad, K., Knobeloch, K.P., Maqbool, M.A., Cauchy, P., Andrau, J.C., Avots, A., Talora, C., Ellenrieder, V., et al. (2016). A threshold level of NFATc1 activity facilitates thymocyte differentiation and opposes notch-driven leukaemia development. Nat Commun 7, 11841.

Alrefai, H., Muhammad, K., Rudolf, R., Pham, D.A., Klein-Hessling, S., Patra, A.K., Avots, A., Bukur, V., Sahin, U., Tenzer, S., et al. (2016). NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells. Nat Commun 7, 11724.

Distler, E., Albrecht, J., Brunk, A., Khan, S., Schnurer, E., Frey, M., Mottok, A., Jordan-Garrote, A.L., Brede, C., Beilhack, A., et al. (2016). Patient-individualized CD8(+) cytolytic T-cell therapy effectively combats minimal residual leukemia in immunodeficient mice. Int J Cancer 138, 1256-1268.

Hesbacher, S., Pfitzer, L., Wiedorfer, K., Angermeyer, S., Borst, A., Haferkamp, S., Scholz, C.J., Wobser, M., Schrama, D., and Houben, R. (2016). RB1 is the crucial target of the Merkel cell polyomavirus Large T antigen in Merkel cell carcinoma cells. Oncotarget 7, 32956-32968.

Monzon-Casanova, E., Rudolf, R., Starick, L., Muller, I., Sollner, C., Muller, N., Westphal, N., Miyoshi-Akiyama, T., Uchiyama, T., Berberich, I., et al. (2016). The Forgotten: Identification and Functional Characterization of MHC Class II Molecules H2-Eb2 and RT1-Db2. J Immunol 196, 988-999.

Schrama, D., Hesbacher, S., Angermeyer, S., Schlosser, A., Haferkamp, S., Aue, A., Adam, C., Weber, A., Schmidt, M., and Houben, R. (2016). Serine 220 phosphorylation of the Merkel cell polyomavirus large T antigen crucially supports growth of Merkel cell carcinoma cells. Int J Cancer 138, 1153-1162.