Structural basis of TRPC4 regulation by calmodulin and pharmacological agents
11/25/2020
Structural basis of TRPC4 regulation by calmodulin and pharmacological agents
Deivanayagabarathy Vinayagam, Dennis Quentin, Jing Yu-Strzelczyk, Oleg Sitsel, Felipe Merino, Markus Stabrin, Oliver Hofnagel, Maolin Yu, Mark W Ledeboer, Georg Nagel, Goran Malojcic, Stefan Raunser
eLife 2020;9:e60603
Canonical transient receptor potential channels (TRPC) are involved in receptor-operated and/or store-operated Ca2+ signaling. Inhibition of TRPCs by small molecules was shown to be promising in treating renal diseases. In cells, the channels are regulated by calmodulin (CaM). Molecular details of both CaM and drug binding have remained elusive so far. Here, we report structures of TRPC4 in complex with three pyridazinone-based inhibitors and CaM. The structures reveal that all the inhibitors bind to the same cavity of the voltage-sensing-like domain and allow us to describe how structural changes from the ligand-binding site can be transmitted to the central ion-conducting pore of TRPC4. CaM binds to the rib helix of TRPC4, which results in the ordering of a previously disordered region, fixing the channel in its closed conformation. This represents a novel CaM-induced regulatory mechanism of canonical TRP channels.
